Composite

Part:BBa_K4836013

Designed by: A Adithya Hitesh, M V P S Sriyanth, Sanchi Chaware, G Manonmani, Dhruvi Panchal, Akhshaya Mohan   Group: iGEM23_IISER-Tirupati-India   (2023-10-10)

LasR expression cassette

This cassette consists of LasR transcription factor that binds to serotonin and TagBFP. A T2A self-cleaving peptide linker sequence was placed between these coding sequences to separate the two proteins during translation within the same operon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None